What is ICSI Treatment?

Intracytoplasmic sperm injection 

Intracytoplasmic sperm injection (ICSI)is a specialised form of In Vitro Fertilisation (IVF) in which a single sperm is injected directly into the egg for fertilisation.

Before a man’s sperm can fertilize a woman’s egg, the head of the sperm must attach to the outside of the egg. Once attached, the sperm pushes through the outer layer to the inside of the egg (cytoplasm), where fertilization takes place.

What is ICSI and how is it done?

In males, sperm is made in the testicles. It makes its way through the reproductive system and combines with the seminal duct fluid. The sperm and this fluid combine to form semen, which is the thick, white ejaculate that emerges from the penis. When there is severe male factor infertility such as- very low sperm count (also known as oligospermia), abnormally shaped sperm (also known as teratozoospermia), poor sperm movement (also known as asthenozoospermia); ICSI is performed.

In this process, the egg from the female is put in a culture plate with numerous sperm from the male, and fertilisation happens when one sperm enters the egg spontaneously during traditional IVF. ICSI is a safe and successful method for couples with male factor infertility, and can enhance the chances of fertilisation for couples who had poor fertilisation in a prior IVF round.

Why ICSI is done? »

When a man’s sperm is unable to fertilise an egg despite IVF, a specialised treatment known as Intracytoplasmic Sperm Injection ICSI is used. This procedure improves the couple’s chances of becoming pregnant. Male infertility caused by low sperm count, low motility, azoospermia, erectile dysfunction, or infertility with no clear cause may be treated using this treatment.

  • It aids in the treatment of infertility issues such as:
    • Artificial insemination (intrauterine insemination [IUI]) or IVF are not possible since the male spouse generates insufficient sperm.
    • It’s possible that the sperm won’t migrate normally.
    • It’s possible that the sperm will have problems adhering to the egg.
    • Sperm may not be able to leave the male reproductive system due to a blockage.
    • Traditional IVF has failed to fertilise eggs, regardless of sperm quality.
    • Eggs that have been developed in vitro are being used.
    • Eggs that had previously been frozen are being utilised.
ICSI Procedure »

In a general IVF procedure, the female partner needs to take fertility medicine to increase egg production in the ovaries. During this period, the doctor will keep an eye on the eggs’ progress. When the egg is ready to be extracted, the ICSI process is carried out.

The following steps are followed in the ICSI procedure-

  • Retrieval of sperm and egg
    Either the male partner must generate a sperm sample by ejaculating into a cup, or the sperm must be surgically extracted. The sperm can be extracted in a variety of different methods. Through a treatment named TESA, sperm is retrieved for ICSI. It is performed under anaesthetic in the operating room or office, and it is timed to coincide with the egg retrieval of their female spouse. The testicle is pierced with a needle, and tissue/sperm are aspirated.
    A small needle and ultrasound probe will be used to extract several eggs from the woman’s ovaries.
  • Sperm Injection into the Egg
    The semen sample is then cleansed and a single sperm is extracted. With a very small hollow needle, this is then inserted into an egg. The sperm does not have to swim through the cervical fluid since it is injected immediately. It can take up to 24 hours for sperm to fertilise an egg and form an embryo.
  • Monitoring the Embryo
    The fertilised embryos are preserved in a laboratory for up to 6 days after fertilisation. During this stage, they are watched for signs of development and growth. The fertilised embryos will not all be transported to the uterus of the mother. As a result, this phase is critical for selecting the healthiest and most viable embryos.
  • Embryo Transfer
    One or two embryos are chosen and transferred to the female partner’s womb using an ultrasound-guided catheter once the embryo has reached a particular stage of development. This might happen two days after conception or five days afterwards.
    The rest of the procedure is similar to that of a biological pregnancy. The embryo must adhere to the uterus’s walls and continue to grow. Two weeks after the fertilised egg has been transferred to the womb, a pregnancy test is typically recommended.
Why Choose Sunrise IVF? »

We understand your dreams of expanding your family and that undergoing infertility treatment may seem like a taxing processes. At Sunrise IVF, our excellent doctors are equipped to help you every step of the way, and to ensure that you have seamless stepping stones onto parenthood.

  • You will be putting your trust in the able hands of our doctors who have notched one of the highest IVF success rates in India, with 1,000+ successful IVF pregnancies.
  • You will be in the care of highly specialized doctors trained in using the best-in-class technology to increase your chances of having a healthy baby.
  • You will be able to attain your parental aspirations sooner as it is our endeavor to ensure that you are able to conceive with the least possible number of ART cycles.
  • Your case is unique and we have solutions specific to your needs – this includes utilizing donor eggs and/or sperms. We’ve got your back.
Do’s & Don’ts »


  • Keep an Eye on What You Eat and Drink– What one consumes has an impact on sperm count and delivery. As a result, it’s important to see their doctor at least three months before the scheduled IVF/ICSI procedure and acquire a recommended diet chart to assist the boost of fertility.
  • Concentrate on Proteins– Protein is regarded as a superfood that can help improve one’s overall health as well as the quality of your sperm. This will ensure that one can offer the best sample possible on the day your IVF process begins.


  • Reduce intake of alcohol– One of the recommended methods to aid the process along is to refrain from alcohol for at least three months before the intended IVF treatment.
  • Quit Smoking– Numerous scientific research and data show that smoking has a negative influence on all elements of the human body. However, the consequences for one’s reproductive health are the most serious and frequently irreversible. While stopping smoking before one’s treatment may seem like too little, every little bit helps.
Cost of IVF »

ICSI cost in India can vary depending on the clinic. At Suunrise IVF, the base price for the treatment remains the same however, a variable cost may be associated with the procedure due to medication used. The total IVF treatment cost can also depend on any additional surgical procedures that need to be performed.

In most cases, the minimum cost ranges between Rs. 2,40,000 to Rs. 2,50,000 per cycle. With a higher budget one can opt for the high-tech assistive technology which will have some extra costs like Rs.1,45,000 to Rs.2,51,000 on the basic costs.

FAQs »
  • What is the difference between IVF and ICSI?
    The way the sperm fertilises the egg is the main difference between IVF and ICSI. The egg and sperm (of which there are many) are left in a petri dish to fertilise on their own during IVF. In ICSI, one sperm is directly injected into the egg.
  • How ICSI can help in infertility?
    ICSI is a IVF that is used primarily for the treatment of severe cases of male-factor infertility. ICSI involves the injection of a single sperm directly into a mature egg.
  • What causes ICSI failure?
    The inability of the sperm to activate the oocyte is the most common reason for unsuccessful fertilisation following ICSI utilising round-head sperm. Fertilization failure can also be caused by nuclear decondensation arrest and/or premature chromosomal condensation in some types of globozoospermia.

Why choose Sunrise IVF?

Specialized center for Infertility Treatment

Sunris IVF Centre, Bareilly can help you achieve your dream of Parenthood

We provide the most comprehensive range of services to cover all the requirements at a Fertility clinic including in-house blood lab, consultations & treatments.

Speak to our Fertility Advisors, Now!

+91 8630268144
The Best IVF center in Bareilly


Specialized center for Infertility Treatment

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